Fibrillarin Rabbit Polyclonal Antibody
cat.: ER1802-81
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Fibrillarin aa 272-321 / 321. |
| Positive control: | Hela cell lysate, Hep G2 cell lysate, 293T cell lysate, NIH-3T3 cell lysate, HL-60, human tonsil tissue, mouse liver tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IHC-P IF-Tissue |
1:500-1:5,000 1:50-1:100 1:200-1:1,000 1:50-1:1,000 |
| Uniprot #: | SwissProt: P22087 Human | P35550 Mouse |
| Alternative names: | 34 kD nucleolar scleroderma antigen 34 kDa nucleolar scleroderma antigen Fbl FBRL_HUMAN FIB FIB1 FLRN Histone-glutamine methyltransferase Nop1p RNA U3 small nucleolar interacting protein 1 RNU3IP1 rRNA 2' O methyltransferase fibrillarin rRNA 2'-O-methyltransferase fibrillarin |
Images
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Fig1:
Western blot analysis of Fibrillarin on different lysates with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: 293T (Human embryonic kidney cell) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-81, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 34 kDa Observed band size: 37 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-81) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Fibrillarin with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-81, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling Fibrillarin with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-81, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.