Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 155 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide of C-terminal human ASK1. |
Positive control: | Human skeletal muscle tissue lysate, human colon cancer tissue, mouse testis tissue, PANC-1. |
Subcellular location: | Cytoplasm, Endoplasmic reticulum. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: Q99683 Human | O35099 Mouse |
Alternative names: | Apoptosis signal regulating kinase 1 Apoptosis signal-regulating kinase 1 ASK 1 ASK-1 ASK1 M3K5 M3K5_HUMAN MAP/ERK kinase kinase 5 MAP3K5 MAPK/ERK kinase kinase 5 MAPKKK5 MEK kinase 5 MEKK 5 MEKK5 Mitogen activated protein kinase kinase kinase 5 Mitogen-activated protein kinase kinase kinase 5 |
Fig1: Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution. | |
Fig2: ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin. |