NQO1 Rabbit Polyclonal Antibody
cat.: ER1802-85
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NQO1 aa 1-270. |
| Positive control: | Siha cell lysate, A549 cell lysate, Siha cell. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P15559 Human | Q64669 Mouse | P05982 Rat |
| Alternative names: | Azoreductase Cytochrome b 5 reductase DHQU DIA 4 DIA4 Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) Diaphorase (NADH/NADPH) Diaphorase 4 Dioxin inducible 1 DT diaphorase DT-diaphorase DTD Menadione reductase NAD(P)H dehydrogenase [quinone] 1 NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible NAD(P)H quinone dehydrogenase 1 NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 NAD(P)H:menadione oxidoreductase 1 NAD(P)H:Quinone acceptor oxidoreductase type 1 NAD(P)H:quinone oxidoreductase 1 NAD(P)H:quinone oxireductase NMOR 1 NMOR I NMOR1 NMORI NQO 1 NQO1 NQO1_HUMAN Phylloquinone reductase QR 1 QR1 Quinone reductase 1 |
Images
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Fig1:
Western blot analysis of NQO1 on different lysates with Rabbit anti-NQO1 antibody (ER1802-85) at 1/1,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: HepG2 (Human liver cancer cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 5 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-85, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 31 kDa Observed band size: 31 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.