Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 31 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human NQO1 aa 1-270. |
Positive control: | Siha cell lysate, A549 cell lysate, Siha cell. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
Uniprot #: | SwissProt: P15559 Human | Q64669 Mouse | P05982 Rat |
Alternative names: | Azoreductase Cytochrome b 5 reductase DHQU DIA 4 DIA4 Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) Diaphorase (NADH/NADPH) Diaphorase 4 Dioxin inducible 1 DT diaphorase DT-diaphorase DTD Menadione reductase NAD(P)H dehydrogenase [quinone] 1 NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible NAD(P)H quinone dehydrogenase 1 NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 NAD(P)H:menadione oxidoreductase 1 NAD(P)H:Quinone acceptor oxidoreductase type 1 NAD(P)H:quinone oxidoreductase 1 NAD(P)H:quinone oxireductase NMOR 1 NMOR I NMOR1 NMORI NQO 1 NQO1 NQO1_HUMAN Phylloquinone reductase QR 1 QR1 Quinone reductase 1 |
Fig1:
Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control: Lane 1: Siha cell lysate Lane 2: A549 cell lysate |
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Fig2: Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |