Connexin 43 Rabbit Polyclonal Antibody
cat.: ER1802-88
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Connexin 43. |
| Positive control: | Mouse testis tissue lysate, human skin tissue, human heart tissue, mouse brain tissue, SH-SY-5Y. |
| Subcellular location: | Plasma membrane. Endoplasmic reticulum. Gap junction. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P17302 Human | P23242 Mouse |
| Alternative names: | Connexin 43 Connexin-43 Cx 43 Cx43 CXA1_HUMAN DFNB38 Gap junction 43 kDa heart protein Gap junction alpha-1 protein Gap junction protein alpha 1 43kDa (connexin 43) Gap junction protein alpha 1 43kDa Gap junction protein alpha like GJA 1 Gja1 GJAL ODD ODDD ODOD SDTY3 |
Images
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Fig1: Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of SH-SY-5Y cells with Connexin 43 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.