RBX1 Rabbit Polyclonal Antibody
cat.: ER1802-9
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 12 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RBX1 aa 1-108 / 108.
Positive control: HepG2 cell lysate, 293T, MCF-7, human colon cancer tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P62877 Human | P62878 Mouse
Alternative names: BA554C12.1 E3 ubiquitin-protein ligase RBX1 FLJ60363 MGC13357 MGC1481 OTTHUMP00000028983 Protein ZYP Rbx 1 Rbx1 RBX1_HUMAN Regulator of cullins 1 Ring box 1 Ring box 1 E3 ubiquitin protein ligase RING box protein 1 RING finger protein 75 RING finger protein RING-box protein 1 Ringbox protein 1 RNF 75 RNF75 ROC 1 ZYP protein
Images
ER1802-9_1.jpg Fig1: Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.
ER1802-9_2.jpg Fig2: ICC staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-9_3.jpg Fig3: ICC staining RBX1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-9_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
ER1802-9_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
ER1802-9_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
ER1802-9_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
ER1802-9_8.jpg Fig8: Flow cytometric analysis of MCF-7 cells with RBX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.