RBX1 Rabbit Polyclonal Antibody
cat.: ER1802-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RBX1 aa 1-108 / 108. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, 293T, MCF-7, human colon cancer tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P62877 Human | P62878 Mouse |
| Alternative names: | BA554C12.1 E3 ubiquitin-protein ligase RBX1 FLJ60363 MGC13357 MGC1481 OTTHUMP00000028983 Protein ZYP Rbx 1 Rbx1 RBX1_HUMAN Regulator of cullins 1 Ring box 1 Ring box 1 E3 ubiquitin protein ligase RING box protein 1 RING finger protein 75 RING finger protein RING-box protein 1 Ringbox protein 1 RNF 75 RNF75 ROC 1 ZYP protein |
Images
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Fig1:
Western blot analysis of RBX1 on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-RBX1 antibody (ER1802-9) at 1/100 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 5 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-9, 1/100 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
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Fig2: ICC staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining RBX1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RBX1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RBX1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.