| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Kv43 aa 435-484 / 655. |
| Positive control: | MCF-7 cell lysate, mouse marrow tissue lysate, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UK17 Human | Q9Z0V1 Mouse |
| Alternative names: | KCND 3 KCND 3L KCND 3S Kcnd3 KCND3_HUMAN KCND3L KCND3S KSHIVB Kv 4.3 MGC14203 MGC142037 OTTHUMP00000063948 Potassium ioinic channel Kv4.3 Potassium voltage gated channel Shal related subfamily member 3 Potassium voltage gated channel subfamily D member 3 Potassium voltage-gated channel subfamily D member 3 Sha1 related potassium channel Kv4.3 Voltage gated K+ channel Voltage gated potassium channel Kv4.3 Voltage gated potassium channel subunit Kv4.3 Voltage-gated potassium channel subunit Kv4.3 |
|
Fig1:
Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Mouse marrow tissue lysate |
|
Fig2: ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
|
Fig8: Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |