KV4.3 Rabbit Polyclonal Antibody
cat.: ER1802-91
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Kv43 aa 435-484 / 655. |
| Positive control: | MCF-7 cell lysate, mouse marrow tissue lysate, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UK17 Human | Q9Z0V1 Mouse |
| Alternative names: | KCND 3 KCND 3L KCND 3S Kcnd3 KCND3_HUMAN KCND3L KCND3S KSHIVB Kv 4.3 MGC14203 MGC142037 OTTHUMP00000063948 Potassium ioinic channel Kv4.3 Potassium voltage gated channel Shal related subfamily member 3 Potassium voltage gated channel subfamily D member 3 Potassium voltage-gated channel subfamily D member 3 Sha1 related potassium channel Kv4.3 Voltage gated K+ channel Voltage gated potassium channel Kv4.3 Voltage gated potassium channel subunit Kv4.3 Voltage-gated potassium channel subunit Kv4.3 |
Images
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Fig1:
Western blot analysis of KV4.3 on different lysates with Rabbit anti-KV4.3 antibody (ER1802-91) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-91, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 73 kDa Observed band size: 72 kDa |
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Fig2: ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.