KV4.3 Rabbit Polyclonal Antibody
cat.: ER1802-91
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Kv43 aa 435-484 / 655.
Positive control: MCF-7 cell lysate, mouse marrow tissue lysate, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1,000
1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UK17 Human | Q9Z0V1 Mouse
Alternative names: KCND 3 KCND 3L KCND 3S Kcnd3 KCND3_HUMAN KCND3L KCND3S KSHIVB Kv 4.3 MGC14203 MGC142037 OTTHUMP00000063948 Potassium ioinic channel Kv4.3 Potassium voltage gated channel Shal related subfamily member 3 Potassium voltage gated channel subfamily D member 3 Potassium voltage-gated channel subfamily D member 3 Sha1 related potassium channel Kv4.3 Voltage gated K+ channel Voltage gated potassium channel Kv4.3 Voltage gated potassium channel subunit Kv4.3 Voltage-gated potassium channel subunit Kv4.3
Images
ER1802-91_1.jpg Fig1: Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Mouse marrow tissue lysate
ER1802-91_2.jpg Fig2: ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-91_3.jpg Fig3: ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-91_4.jpg Fig4: ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-91_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
ER1802-91_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
ER1802-91_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
ER1802-91_8.jpg Fig8: Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.