Fibulin 5 Rabbit Polyclonal Antibody
cat.: ER1802-95
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Fibulin aa 203-378 / 448. |
| Positive control: | Human skin tissue, JAR, SiHa, SKOV-3, rat testis tissue, mouse ovary tissue, Hela. |
| Subcellular location: | Extracellular matrix. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UBX5 Human | Q9WVH9 Mouse | Q9WVH8 Rat |
| Alternative names: | ADCL2 ARCL1A ARMD3 Dance Developmental arteries and neural crest EGF like protein Developmental arteries and neural crest EGF-like protein EVEC Fbln5 FBLN5_HUMAN FIBL 5 FIBL-5 Fibulin-5 FLJ90059 UP50 Urine p50 protein |
Images
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Fig1: ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunocytochemistry analysis of Hela cells labeling Fibulin 5 with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
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Fig7:
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.2 kDa Observed band size: 50 kDa |
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Fig8:
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution. Lane 1: SK-OV-3 (human ovarian cancer cells) cell tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.2 kDa Observed band size: 50 kDa |
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Fig9:
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution. Lane 1: JAR (human choriocarcinoma cells) cell tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.2 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.