Glutathione Reductase Rabbit Polyclonal Antibody
cat.: ER1802-96
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GLUR aa 273-446 / 522. |
| Positive control: | A549, rat liver tissue, rat epididymis tissue, human liver tissue, human thyroid gland tissue. |
| Subcellular location: | Cytoplasm. Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P00390 Human | P70619 Rat |
| Alternative names: | GLR1 GLUR Glutathione reductase Glutathione reductase mitochondrial Glutathione reductase, mitochondrial GR Gr1 GRase GRD 1 GRD1 GSHR_HUMAN GSR MGC78522 |
Images
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Fig1:
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (ER1802-96) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Glutathione Reductase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-96) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution. Positive control: Lane 1: A549 Lane 2: Rat liver tissue |
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Fig3: ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLUR antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A549 cells with GLUR antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.