Anti-Bcl2 antibody
cat.: ER1802-97
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 22/26 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human BCL-2 20-100 aa.
Positive control: HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.
Subcellular location: Mitochondrion. Endoplasmic reticulum. Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:200-1:500
1:50-1:200
1:50-1:100
Uniprot #: P10415(Human) P10417(Mouse)
Alternative names: Apoptosis regulator Bcl 2 antibody Apoptosis regulator Bcl-2 antibody Apoptosis regulator Bcl2 antibody AW986256 antibody B cell CLL/lymphoma 2 antibody B cell leukemia/lymphoma 2 antibody Bcl-2 antibody Bcl2 antibody BCL2_HUMAN antibody C430015F12Rik antibody D630044D05Rik antibody D830018M01Rik antibody Leukemia/lymphoma, B-cell, 2 antibody Oncogene B-cell leukemia 2 antibody PPP1R50 antibody Protein phosphatase 1, regulatory subunit 50 antibody
Images
ER1802-97_1.jpg Fig1: Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon
ER1802-97_2.jpg Fig2: ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-97_3.jpg Fig3: ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-97_4.jpg Fig4: ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-97_5.jpg Fig5: Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.