ERGI3 Rabbit Polyclonal Antibody
cat.: ER1802-98
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ERGI3 aa 334-383 / 383. |
| Positive control: | Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, mouse placenta tissue lysate, mouse bone marrow tissue lysate, rat placenta tissue lysate, rat bone marrow tissue lysate, LOVO, SiHa, human colon cancer tissue, human placenta tissue, human uterus tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum. Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y282 Human | Q9CQE7 Mouse |
| Alternative names: | 2310015B14Rik AV318804 C20orf47 CGI 54 D2Ucla1 dJ477O4.2 DKFZp547A2190 Endoplasmic reticulum Golgi intermediate compartment protein 3 endoplasmic reticulum localized protein ERp43 Endoplasmic reticulum-Golgi intermediate compartment protein 3 ERGI3_HUMAN ERGIC and golgi 3 ergic3 ERV46 NY BR 84 PRO0989 RP23-220D12.2 RP3-477O4.1 SDBCAG84 Serologically defined breast cancer antigen 84 Serologically defined breast cancer antigen NY BR 84 Serologically defined breast cancer antigen NY-BR-84 |
Images
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Fig1:
Western blot analysis of ERGI3 on different lysates with Rabbit anti-ERGI3 antibody (ER1802-98) at 1/5,000 dilution. Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: Mouse placenta tissue lysate Lane 4: Mouse bone marrow tissue lysate Lane 5: Rat placenta tissue lysate Lane 6: Rat bone marrow tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-98, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 54 kDa |
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Fig2: ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.