ERGI3 Rabbit Polyclonal Antibody
cat.: ER1802-98
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ERGI3 aa 334-383 / 383. |
| Positive control: | Siha, human placenta tissue, rat bone marrow tissue, LOVO, human colon cancer tissue, human uterus tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum. Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y282 Human | Q9CQE7 Mouse |
| Alternative names: | 2310015B14Rik AV318804 C20orf47 CGI 54 D2Ucla1 dJ477O4.2 DKFZp547A2190 Endoplasmic reticulum Golgi intermediate compartment protein 3 endoplasmic reticulum localized protein ERp43 Endoplasmic reticulum-Golgi intermediate compartment protein 3 ERGI3_HUMAN ERGIC and golgi 3 ergic3 ERV46 NY BR 84 PRO0989 RP23-220D12.2 RP3-477O4.1 SDBCAG84 Serologically defined breast cancer antigen 84 Serologically defined breast cancer antigen NY BR 84 Serologically defined breast cancer antigen NY-BR-84 |
Images
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Fig1:
Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution. Positive control: Lane 1: SiHa Lane 2: Human placenta Lane 3: Rat bone marrow |
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Fig2: ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.