ERGI3 Rabbit Polyclonal Antibody
cat.: ER1802-98
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ERGI3 aa 334-383 / 383.
Positive control: Siha, human placenta tissue, rat bone marrow tissue, LOVO, human colon cancer tissue, human uterus tissue, mouse brain tissue.
Subcellular location: Endoplasmic reticulum. Golgi apparatus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9Y282 Human | Q9CQE7 Mouse
Alternative names: 2310015B14Rik AV318804 C20orf47 CGI 54 D2Ucla1 dJ477O4.2 DKFZp547A2190 Endoplasmic reticulum Golgi intermediate compartment protein 3 endoplasmic reticulum localized protein ERp43 Endoplasmic reticulum-Golgi intermediate compartment protein 3 ERGI3_HUMAN ERGIC and golgi 3 ergic3 ERV46 NY BR 84 PRO0989 RP23-220D12.2 RP3-477O4.1 SDBCAG84 Serologically defined breast cancer antigen 84 Serologically defined breast cancer antigen NY BR 84 Serologically defined breast cancer antigen NY-BR-84
Images
ER1802-98_1.jpg Fig1: Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: Human placenta
Lane 3: Rat bone marrow
ER1802-98_2.jpg Fig2: ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-98_3.jpg Fig3: ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1802-98_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
ER1802-98_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
ER1802-98_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
ER1802-98_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
ER1802-98_8.jpg Fig8: Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.