LAMP2a Rabbit Polyclonal Antibody
cat.: ER1802-99
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human LAMP2a. |
| Positive control: | Mouse placenta tissue, human prostate tissue, human kidney tissue, human placenta tissue. |
| Subcellular location: | Plasma membrane. Endosome. Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:2000 1:50-1:200 |
| Uniprot #: | SwissProt: P13473 Human |
| Alternative names: | CD107 antigen-like family member B CD107b LAMP 2 Lamp 2a LAMP-2 LAMP2 LAMP2_HUMAN Lysosome-associated membrane glycoprotein 2 Lysosome-associated membrane protein 2 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LAMP2a antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-LAMP2a antibody. Counter stained with hematoxylin. |
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Fig4:
Western blot analysis of LAMP2a on different lysates with Rabbit anti-LAMP2a antibody (ER1802-99) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (20 μg/Lane) Lane 2: Mouse liver tissue lysate (40 μg/Lane) Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1802-99, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 70-140 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.