UAP1 Rabbit Polyclonal Antibody
cat.: ER1803-01
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human UAP1 aa 392-441 / 522. |
| Positive control: | SiHa cell lysates, 293T, SiHa, rat brain tissue, human liver tissue, K562. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16222 Human |
| Alternative names: | AGX 1 AGX AGX-1 AGX-2 AGX1 Antigen X AntigenX SPAG 2 SPAG2 Sperm associated antigen 2 Sperm-associated antigen 2 UAP 1 uap1 UAP1_HUMAN UDP N acetylhexosamine pyrophosphorylase UDP N acteylglucosamine pyrophosphorylase 1 UDP-N-acetylglucosamine pyrophosphorylase |
Images
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Fig1:
Western blot analysis of UAP1 on SiHa cell lysates with Rabbit anti-UAP1 antibody (ER1803-01) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-01) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining UAP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining UAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of K562 cells with UAP1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.