UAP1 Rabbit Polyclonal Antibody
cat.: ER1803-02
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human UAP1 aa 33-82 / 522. |
| Positive control: | 293T, A431, SiHa, rat brain tissue, human liver tissue, human prostate cancer tissue, mouse testis tissue, K562. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16222 Human | Q91YN5 Mouse |
| Alternative names: | AGX 1 AGX AGX-1 AGX-2 AGX1 Antigen X AntigenX SPAG 2 SPAG2 Sperm associated antigen 2 Sperm-associated antigen 2 UAP 1 uap1 UAP1_HUMAN UDP N acetylhexosamine pyrophosphorylase UDP N acteylglucosamine pyrophosphorylase 1 UDP-N-acetylglucosamine pyrophosphorylase |
Images
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Fig1: ICC staining UAP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining UAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining UAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP1 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of K562 cells with UAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.