UAP1 Rabbit Polyclonal Antibody
cat.: ER1803-02
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human UAP1 aa 33-82 / 522.
Positive control: 293T, A431, SiHa, rat brain tissue, human liver tissue, human prostate cancer tissue, mouse testis tissue, K562.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q16222 Human | Q91YN5 Mouse
Alternative names: AGX 1 AGX AGX-1 AGX-2 AGX1 Antigen X AntigenX SPAG 2 SPAG2 Sperm associated antigen 2 Sperm-associated antigen 2 UAP 1 uap1 UAP1_HUMAN UDP N acetylhexosamine pyrophosphorylase UDP N acteylglucosamine pyrophosphorylase 1 UDP-N-acetylglucosamine pyrophosphorylase
Images
ER1803-02_1.jpg Fig1: ICC staining UAP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-02_2.jpg Fig2: ICC staining UAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-02_3.jpg Fig3: ICC staining UAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-02_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
ER1803-02_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
ER1803-02_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
ER1803-02_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
ER1803-02_8.jpg Fig8: Flow cytometric analysis of K562 cells with UAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.