Kir3.4 Rabbit Polyclonal Antibody
cat.: ER1803-03
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Kir3.4. |
| Positive control: | Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P48544 Human | P48545 Mouse | P48548 Rat |
| Alternative names: | Cardiac ATP sensitive potassium channel Cardiac inward rectifier CIR G protein activated inward rectifier potassium channel 4 G protein-activated inward rectifier potassium channel 4 GIRK 4 GIRK-4 GIRK4 Heart KATP channel Inward rectifier K(+) channel Kir3.4 Inward rectifier K+ channel KIR3.4 Inward rectifier potassium channel KIR3.4 inwardly rectifying subfamily J member 5 IRK-4 IRK5_HUMAN KATP 1 KATP-1 KATP1 KCNJ 5 Kcnj5 KIR 3.4 KIR3.4 LQT13 Potassium channel Potassium channel inwardly rectifying subfamily J member 5 Potassium inwardly rectifying channel J5 Potassium inwardly rectifying channel subfamily J member 5 potassium voltage-gated channel subfamily J member 5 |
Images
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Fig1:
Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Rat heart |
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Fig2: ICC staining Kir3.4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Kir3.4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Kir3.4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of HepG2 cells with Kir3.4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.