Kir3.4 Rabbit Polyclonal Antibody
cat.: ER1803-03
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.4ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Kir3.4. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, Human Adrenal gland tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: P48544 Human | P48545 Mouse | P48548 Rat |
| Alternative names: | Cardiac ATP sensitive potassium channel Cardiac inward rectifier CIR G protein activated inward rectifier potassium channel 4 G protein-activated inward rectifier potassium channel 4 GIRK 4 GIRK-4 GIRK4 Heart KATP channel Inward rectifier K(+) channel Kir3.4 Inward rectifier K+ channel KIR3.4 Inward rectifier potassium channel KIR3.4 inwardly rectifying subfamily J member 5 IRK-4 IRK5_HUMAN KATP 1 KATP-1 KATP1 KCNJ 5 Kcnj5 KIR 3.4 KIR3.4 LQT13 Potassium channel Potassium channel inwardly rectifying subfamily J member 5 Potassium inwardly rectifying channel J5 Potassium inwardly rectifying channel subfamily J member 5 potassium voltage-gated channel subfamily J member 5 |
Images
|
Fig1:
Western blot analysis of Kir3.4 on different lysates with Rabbit anti-Kir3.4 antibody (ER1803-03) at 1/500 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-03, 1/500 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 37 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Adrenal gland Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ER1803-03, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.