macroH2A.1 Rabbit Polyclonal Antibody
cat.: ER1803-04
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human macroH2A.1 aa 190-369. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, MDA-MB-231 cell lysate, human colon tissue, human placenta tissue, mouse brain tissue, SiHa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O75367 Human | Q9QZQ8 Mouse |
| Alternative names: | Core histone macro h2a.1 Core histone macro-H2A.1 H2A histone family member Y H2A.y H2A/y H2AF12M H2AFJ H2afy H2AY_HUMAN Histone H2A.Y Histone macroH2A1 Histone macroH2A1.1 Histone macroH2A1.2 Macroh2a1 MACROH2A1.1 MacroH2A1.2 Medulloblastoma antigen MU MB 50.205 Medulloblastoma antigen MU-MB-50.205 mH2a mH2A1 |
Images
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Fig1:
Western blot analysis of macroH2A.1 on different lysates with Rabbit anti-macroH2A.1 antibody (ER1803-04) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: MDA-MB-231 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of SiHa cells with macroH2A.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.