Tyk2 Rabbit Polyclonal Antibody
cat.: ER1803-05
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 134 kDa
Isotype: IgG
Immunogen: Recombinant protein within human TYK2 aa 390-620.
Positive control: Raji cell lysate, Jurkat cell lysate, A549 cell lysate, MCF-7, human liver carcinoma tissue, human colon tissue, rat kidney tissue.
Subcellular location: Cytoskeleton. Nucleus. Cytosol. Plasma membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P29597 Human | Q9R117 Mouse
Alternative names: JTK 1 JTK1 Non receptor tyrosine protein kinase 2 Non receptor tyrosine protein kinase TYK2 Non-receptor tyrosine-protein kinase TYK2 OTTHUMP00000232745 OTTHUMP00000232746 OTTHUMP00000232748 Protein Tyrosine Kinase 2 TYK 2 Tyk2 TYK2_HUMAN Tyrosine kinase 2
Images
ER1803-05_1.jpg Fig1: Western blot analysis of Tyk2 on different lysates with Rabbit anti-Tyk2 antibody (ER1803-05) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A549 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 134 kDa
Observed band size: 134 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-05) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ER1803-05_2.jpg Fig2: ICC staining of Tyk2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1803-05, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1803-05_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Tyk2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-05_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Tyk2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-05_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Tyk2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-05_6.jpg Fig6: Flow cytometric analysis of Tyk2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1803-05, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.