Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 53 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human SERPINC1 aa 150-370 / 464. |
Positive control: | HepG2, mouse smooth muscle tissue, mouse lung tissue, H22, rat kidney tissue, human liver tissue, human small intestine tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1;1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P01008 Human | P32261 Mouse | Q5M7T5 Rat |
Alternative names: | ANT3_HUMAN Antithrombin Antithrombin III Antithrombin-III AntithrombinIII AT 3 AT III AT3 AT3D ATIII Heparin cofactor I MGC22579 Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 Serine cysteine proteinase inhibitor clade C member 1 Serine proteinase inhibitor clade C member 1 Serpin C1 Serpin family C member 1 Serpin peptidase inhibitor clade C (antithrombin) member 1 SERPINC1 THPH7 |
Fig1:
Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 Lane 2: Mouse smooth muscle Lane 3: Mouse lung |
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Fig2: ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. | |
Fig7: Flow cytometric analysis of HepG2 cells with SERPINC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |