Antithrombin III / ATIII / SERPINC1 Rabbit Polyclonal Antibody
cat.: ER1803-06
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SERPINC1 aa 150-370 / 464. |
| Positive control: | Human plasma lysates, H22, HepG2, rat kidney tissue, human liver tissue, human small intestine tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:50,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P01008 Human | P32261 Mouse | Q5M7T5 Rat |
| Alternative names: | ANT3_HUMAN Antithrombin Antithrombin III Antithrombin-III AntithrombinIII AT 3 AT III AT3 AT3D ATIII Heparin cofactor I MGC22579 Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 Serine cysteine proteinase inhibitor clade C member 1 Serine proteinase inhibitor clade C member 1 Serpin C1 Serpin family C member 1 Serpin peptidase inhibitor clade C (antithrombin) member 1 SERPINC1 THPH7 |
Images
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Fig1:
Western blot analysis of Antithrombin III / ATIII / SERPINC1 on human plasma lysate with Rabbit anti-Antithrombin III / ATIII / SERPINC1 antibody (ER1803-06) at 1/50,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-06, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
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Fig2: ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.