KCNK4 / K2P4.1 Rabbit Polyclonal Antibody
cat.: ER1803-07
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human K2P41 aa 326-375 / 393.
Positive control: Mouse colon tissue lysate, mouse brain tissue lysate, rat colon tissue lysate, rat brain tissue lysate, rat large intestine tissue, mouse colon tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:2,000
1:50-1:200
Uniprot #: SwissProt: Q9NYG8 Human | O88454 Mouse | G3V8V5 Rat
Alternative names: K2p4.1 K2P4.1 potassium channel KCNK 4 MGC144821 Potassium channel subfamily K member 4 Potassium inwardly rectifying channel subfamily K member 4 TRAAK TRAAK1 TRAAKt TWIK related arachidonic acid stimulated potassium channel protein Two pore K(+) channel KT4.1 Two pore K+ channel KT4.1 Two pore potassium channel KT4.1
Images
ER1803-07_1.jpg Fig1: Western blot analysis of KCNK4 / K2P4.1 on different lysates with Rabbit anti-KCNK4 / K2P4.1 antibody (ER1803-07) at 1/2,000 dilution.

Lane 1: Mouse colon tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat colon tissue lysate
Lane 4: Rat brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 50 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1803-07_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
ER1803-07_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.