UQCRC2 Rabbit Polyclonal Antibody
cat.: ER1803-08
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UQCRC2 aa 90-370. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, L-929 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat heart tissue lysate, rat kidney tissue lysate, HepG2, LOVO, SiHa, rat heart tissue, human liver tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P22695 Human | Q9DB77 Mouse | P32551 Rat |
| Alternative names: | Complex III subunit 2 Core protein II Cytochrome b c1 complex subunit 2 mitochondrial Cytochrome b-c1 complex subunit 2 EC 1.10.2.2 MC3DN5 mitochondrial QCR2 QCR2_HUMAN Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial Ubiquinol cytochrome c reductase core protein II Ubiquinol-cytochrome-c reductase complex core protein 2 UQCR2 Uqcrc2 |
Images
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Fig1:
Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (ER1803-08) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: L-929 cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Rat heart tissue lysate (20 µg/Lane) Lane 7: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 48 kDa Observed band size: 40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-08) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). |
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Fig3: ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). |
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Fig4: ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of HepG2 cells with UQCRC2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.