USP13 Rabbit Polyclonal Antibody
cat.: ER1803-09
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, FC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 97 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human USP13 aa 570-776 / 863.
Positive control: 293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.
Subcellular location: Nucleus. Cytosol.
Recommended Dilutions:
  WB
  ICC
  FC
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:100
1:100-1:200
Uniprot #: SwissProt: Q92995 Human | Q5BKP2 Mouse
Alternative names: Deubiquitinating enzyme 13 Isopeptidase T 3 Isopeptidase T-3 Isopeptidase T3 ISOT 3 ISOT-3 ISOT3 Ubiquitin carboxyl terminal hydrolase 13 Ubiquitin carboxyl-terminal hydrolase 13 Ubiquitin specific peptidase 13 (isopeptidase T3) Ubiquitin specific peptidase 13 Ubiquitin specific processing protease 13 Ubiquitin specific protease 13 Ubiquitin thioesterase 13 Ubiquitin thiolesterase 13 Ubiquitin-specific-processing protease 13 UBP13 UBP13_HUMAN USP 13 USP13
Images
ER1803-09_1.jpg Fig1: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain
ER1803-09_2.jpg Fig2: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-09_3.jpg Fig3: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-09_4.jpg Fig4: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.