USP13 Rabbit Polyclonal Antibody
cat.: ER1803-09
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 97 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human USP13 aa 570-776 / 863. |
| Positive control: | 293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63. |
| Subcellular location: | Nucleus. Cytosol. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 1:100-1:200 |
| Uniprot #: | SwissProt: Q92995 Human | Q5BKP2 Mouse |
| Alternative names: | Deubiquitinating enzyme 13 Isopeptidase T 3 Isopeptidase T-3 Isopeptidase T3 ISOT 3 ISOT-3 ISOT3 Ubiquitin carboxyl terminal hydrolase 13 Ubiquitin carboxyl-terminal hydrolase 13 Ubiquitin specific peptidase 13 (isopeptidase T3) Ubiquitin specific peptidase 13 Ubiquitin specific processing protease 13 Ubiquitin specific protease 13 Ubiquitin thioesterase 13 Ubiquitin thiolesterase 13 Ubiquitin-specific-processing protease 13 UBP13 UBP13_HUMAN USP 13 USP13 |
Images
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Fig1:
Western blot analysis of USP13 on different lysates with Rabbit anti-USP13 antibody (ER1803-09) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-USP13 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-09) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Daudi Lane 3: Mouse testis Lane 4: Rat brain |
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Fig3: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.