ApoER2 Rabbit Polyclonal Antibody
cat.: ER1803-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human APOER2 aa 494-722 / 963.
Positive control: Mouse placenat tissue, SH-SY-5Y, F9, HepG2, human placenta tissue.
Subcellular location: Cell membrane. Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50
1:50-1:100
Uniprot #: SwissProt: Q14114 Human | Q924X6 Mouse
Alternative names: APOER2 Apolipoprotein E receptor 2 low density lipoprotein receptor-related protein 8 Low-density lipoprotein receptor-related protein 8 LRP-8 LRP8 LRP8_HUMAN
Images
ER1803-10_1.jpg Fig1: Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse placenta
Lane 2: SH-SY-5Y
ER1803-10_2.jpg Fig2: ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-10_3.jpg Fig3: ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-10_4.jpg Fig4: ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-10_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.
ER1803-10_6.jpg Fig6: Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.