ApoER2 Rabbit Polyclonal Antibody
cat.: ER1803-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human APOER2 aa 494-722 / 963. |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
WB IHC-P IF ELISA |
1:500-1:2,000 1:100-1:500 1:100-1:500 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: Q14114 Human | Q924X6 Mouse Entrez Gene: 362558 Rat |
| Alternative names: | APOER2 Apolipoprotein E receptor 2 low density lipoprotein receptor-related protein 8 Low-density lipoprotein receptor-related protein 8 LRP-8 LRP8 LRP8_HUMAN |
Images
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Fig1:
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ApoER2 Polyclonal Antibody, Unconjugated(ER1803-10) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig2:
Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-ApoER2 (ER1803-10) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5,000 dilution Predicted band size: 102 kD Observed band size: 102 kD |
|
Fig3:
Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Lung (Rat) Lysate at 40 ug Lane 3: U87MG (Human) Lysate at 40 ug Lane 4: Hela (Human) Lysate at 40 ug Primary: Anti-ApoER2 (ER1803-10) at 1/1,000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 170/130 kD Observed band size: 130/125 kD |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.