Orai1 Rabbit Polyclonal Antibody
cat.: ER1803-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Orai1 aa 181-230 / 304. |
| Positive control: | human skin tissue, human esophagus tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96D31 Human | Q8BWG9 Mouse |
| Alternative names: | Calcium release activated calcium channel protein 1 Calcium release activated calcium modulator 1 Calcium release-activated calcium channel protein 1 CRACM 1 CRACM1 CRCM1 CRCM1_HUMAN FLJ14466 Orai 1 ORAI calcium release activated calcium modulator 1 orai1 ORAT1 Protein orai 1 Protein orai-1 TMEM 142A TMEM142A Transmembrane protein 142A |
Images
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Fig1:
Western blot analysis of Orai1 on different lysates with Rabbit anti-Orai1 antibody (ER1803-11) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Orai1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Orai1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.