Orai1 Rabbit Polyclonal Antibody
cat.: ER1803-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Orai1 aa 181-230 / 304. |
| Positive control: | PC-3M, LOVO, MCF-7, human skin tissue, human esophagus tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96D31 Human | Q8BWG9 Mouse |
| Alternative names: | Calcium release activated calcium channel protein 1 Calcium release activated calcium modulator 1 Calcium release-activated calcium channel protein 1 CRACM 1 CRACM1 CRCM1 CRCM1_HUMAN FLJ14466 Orai 1 ORAI calcium release activated calcium modulator 1 orai1 ORAT1 Protein orai 1 Protein orai-1 TMEM 142A TMEM142A Transmembrane protein 142A |
Images
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Fig1: Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution. |
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Fig2: ICC staining Orai1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Orai1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Orai1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Orai1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of MCF-7 cells with Orai1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.