Orai3 Rabbit Polyclonal Antibody
cat.: ER1803-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Orai-3 aa 11-60 / 295. |
| Positive control: | Raji, A549, MG-63, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human kidney tissue, human placenta tissue, mouse testis tissue. |
| Subcellular location: | Membrane. Multi-pass membrane protein. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9BRQ5 Human | Q6P8G8 Mouse | Q6AXR8 Rat |
| Alternative names: | MGC13024 Orai 3 ORAI calcium release activated calcium modulator 3 Orai3 ORAI3_HUMAN Protein orai 3 Protein orai-3 TMEM 142C TMEM142C Transmembrane protein 142C |
Images
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Fig1: ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin. |
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Fig9:
Western blot analysis of Orai3 on different lysates with Rabbit anti-Orai3 antibody (ER1803-12) at 1/500 dilution. Lane 1: MG-63 (Human cervical adenocarcinoma cell) cell lysate Lane 2: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 3: MCF7 (Human breast cancer cells) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast cells) cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at cell:20ug/lane,tissue:40ug/lane. Exposure time: 15 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-12, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 31 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.