| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cacng4 aa 189-238 / 327. |
| Positive control: | MCF-7, SH-SY-5Y, rat testis tissue, rat brain tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UBN1 Human | Q8VHW9 Rat |
| Alternative names: | CACNG4 Calcium channel voltage dependent gamma subunit 4 CCG4_HUMAN Neuronal voltage gated calcium channel gamma 4 subunit Neuronal voltage-gated calcium channel gamma-4 subunit TARP gamma-4 Transmembrane AMPAR regulatory protein gamma-4 Voltage dependent calcium channel gamma 4 subunit Voltage-dependent calcium channel gamma-4 subunit |
|
Fig1: Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution. |
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Fig2: ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |