Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 30 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human NDUFS3 aa 7-239 / 264. |
Positive control: | LOVO, MCF-7, rat epididymis tissue, human liver tissue, mouse brain tissue. |
Subcellular location: | Mitochondrion inner membrane. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:2,000 1:50-1:100 |
Uniprot #: | SwissProt: O75489 Human | Q9DCT2 Mouse | D3ZG43 Rat |
Alternative names: | CI 30 CI 30KD CI-30kD Complex I 30KD Complex I 30kDa subunit COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT Complex I-30kD mitochondrial NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 NADH dehydrogenase ubiquinone 30 kDa subunit NADH-ubiquinone oxidoreductase 30 kDa subunit NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 NDUFS3 NDUS3_HUMAN |
Fig1:
Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution. Positive control: Lane 1: LOVO Lane 2: MCF-7 |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of MCF-7 cells with NDUFS3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |