GPX4 Rabbit Polyclonal Antibody
cat.: ER1803-15
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GPX4 aa 1-197 / 197. |
| Positive control: | Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue. |
| Subcellular location: | Cytoplasm. Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50 -1:600 1:50-1:100 |
| Uniprot #: | SwissProt: P36969 Human | O70325 Mouse | P36970 Rat |
| Alternative names: | Glutathione peroxidase 4 GPX 4 GPX-4 GPX4 GPX4_HUMAN GSHPx-4 MCSP mitochondrial PHGPx Phospholipid hydroperoxidase Phospholipid hydroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase mitochondrial snGPx snPHGPx Sperm nucleus glutathione peroxidase |
Images
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Fig1:
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ER1803-15) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate (20 µg/Lane) Lane 2: Mouse testis tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-15) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.