Anti-GPX4 antibody
cat.: ER1803-15
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human GPX4 aa 1-197 / 197.
Positive control: Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue.
Subcellular location: Cytoplasm. Mitochondrion.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:100
1:50 -1:200
1:50-1:100
Uniprot #: SwissProt: P36969 Human | O70325 Mouse | P36970 Rat
Alternative names: Glutathione peroxidase 4 GPX 4 GPX-4 GPX4 GPX4_HUMAN GSHPx-4 MCSP mitochondrial PHGPx Phospholipid hydroperoxidase Phospholipid hydroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase mitochondrial snGPx snPHGPx Sperm nucleus glutathione peroxidase
Images
ER1803-15_1.jpg Fig1: Western blot analysis of GPX4 on rat testis tissue lysate using anti- GPX4 antibody at 1/500 dilution.
ER1803-15_2.jpg Fig2: ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-15_3.jpg Fig3: ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-15_4.jpg Fig4: ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-15_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.
ER1803-15_6.jpg Fig6: Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.