| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GPX4 aa 1-100 / 197. |
| Positive control: | Rat testis tissue lysate, mouse testis tissue lysate. |
| Subcellular location: | Cytoplasm. Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P36969 Human | O70325 Mouse | P36970 Rat |
| Alternative names: | Glutathione peroxidase 4 GPX 4 GPX-4 GPX4 GPX4_HUMAN GSHPx-4 MCSP mitochondrial PHGPx Phospholipid hydroperoxidase Phospholipid hydroperoxide glutathione peroxidase Phospholipid hydroperoxide glutathione peroxidase mitochondrial snGPx snPHGPx Sperm nucleus glutathione peroxidase |
|
Fig1:
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ER1803-15) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1minutes 2seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-15, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22.2 kDa Observed band size: 22.2 kDa |