WASL Rabbit Polyclonal Antibody
cat.: ER1803-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human WASL aa 89-295 / 505. |
| Positive control: | SH-SY-5Y, SiHa, SK-Br-3, rat brain tissue, human small intestine tissue. |
| Subcellular location: | Cytoplasm. Cytoskeleton. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: O00401 Human | O08816 Rat |
| Alternative names: | WASP family, verprolin homology domain-containing protein 1 WASP family member 1 FLJ31482 homology of dictyostelium scar 1 KIAA0269 Protein WAVE-1 Protein WAVE1 scar, Dictyostelium, homology of, 1 SCAR1 Similar to a plant extensin like protein Verprolin homology domain containing protein 1 Verprolin homology domain-containing protein 1 WAS protein family, member 1 Wasf1 WASF1_HUMAN WASL WASP family 1 WASP family protein member 1 WASP family, verprolin homology domain-containing protein WAVE WAVE1 Wiskott Aldrich syndrome protein family member 1 Wiskott-Aldrich syndrome protein family member 1 |
Images
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Fig1:
Western blot analysis of WASL on different lysates with Rabbit anti-WASL antibody (ER1803-16) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-WASL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-16) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of WASL on SK-BR-3 and SH-SY-5Y cell lysate using anti- WASL antibody at 1/500 dilution. |
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Fig3: ICC staining WASL in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining WASL in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- WASL antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti- WASL antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of SH-SY5Y cells with WASL antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.