Vitamin D3 Receptor Rabbit Polyclonal Antibody
cat.: ER1803-18
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Vitamin D Receptor aa 117-297 / 427. |
| Positive control: | U937, SK-Br-3, LOVO, MCF-7, PC-3M, rat skin tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P11473 Human | P13053 Rat |
| Alternative names: | 1 25 dihydroxyvitamin D3 receptor 1 1,25 dihydroxyvitamin D3 receptor 1,25-@dihydroxyvitamin D3 receptor 25-dihydroxyvitamin D3 receptor Member 1 NR1I1 Nuclear receptor subfamily 1 group I member 1 PPP1R163 Protein phosphatase 1, regulatory subunit 163 VDR VDR_HUMAN Vitamin D (1,25- dihydroxyvitamin D3) receptor Vitamin D hormone receptor Vitamin D nuclear receptor variant 1 Vitamin D receptor Vitamin D3 receptor |
Images
|
Fig1:
Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution. Positive control: Lane 1: U937 Lane 2: SK-Br-3 |
|
Fig2: ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.