Anti-Vitamin D3 Receptor antibody
cat.: ER1803-18
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Vitamin D Receptor aa 90-320
Positive control: U937, SK-Br-3, LOVO, MCF-7, PC-3M, rat skin tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P11473 Human | P13053 Rat
Alternative names: 1 25 dihydroxyvitamin D3 receptor 1 1,25 dihydroxyvitamin D3 receptor 1,[email protected] D3 receptor 25-dihydroxyvitamin D3 receptor Member 1 NR1I1 Nuclear receptor subfamily 1 group I member 1 PPP1R163 Protein phosphatase 1, regulatory subunit 163 VDR VDR_HUMAN Vitamin D (1,25- dihydroxyvitamin D3) receptor Vitamin D hormone receptor Vitamin D nuclear receptor variant 1 Vitamin D receptor Vitamin D3 receptor
Images
ER1803-18_1.jpg Fig1: Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.
Positive control:
Lane 1: U937
Lane 2: SK-Br-3
ER1803-18_2.jpg Fig2: ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-18_3.jpg Fig3: ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-18_4.jpg Fig4: ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-18_5.jpg Fig5: Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.