RYR1 Rabbit Polyclonal Antibody
cat.: ER1803-19
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | Dot Blot, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 565 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RYR1 aa 1,354-1,403 / 5,038. |
| Positive control: | Human fetal skeletal muscle tissue, mouse cerebellum tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, SiHa. |
| Subcellular location: | Sarcoplasmic reticulum membrane. |
| Recommended Dilutions:
Dot Blot IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P21817 Human | E9PZQ0 Mouse | F1LMY4 Rat |
| Alternative names: | Arrhythmogenic right ventricular dysplasia 2 ARVC2 ARVD2 Brain ryanodine receptor calcium release channel Brain type ryanodine receptor Cardiac muscle ryanodine receptor calcium release channel Cardiac muscle type ryanodine receptor CCO Central core disease of muscle HBRR hRYR 2 hRYR2 MHS MHS1 Ryanodine receptor 1 (skeletal) Ryanodine receptor 1 Ryanodine receptor 2 (cardiac) Ryanodine receptor 2 Ryanodine receptor 3 Ryanodine receptor type1 RYDR RYR 1 RYR 2 RYR 3 RYR RYR-1 RyR1 RYR1_HUMAN RYR2 RYR3 Sarcoplasmic reticulum calcium release channel Skeletal muscle calcium release channel Skeletal muscle ryanodine receptor Skeletal muscle type ryanodine receptor Skeletal muscle-type ryanodine receptor SKRR Type 1 like ryanodine receptor Type 1 ryanodine receptor VTSIP |
Images
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Fig1: Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR1 antibody. Counter stained with hematoxylin. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-RYR1 antibody (ER1803-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-RYR1 antibody (ER1803-19) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-19) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of SiHa cells with RYR1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.