DDIT3 Rabbit Polyclonal Antibody
cat.: ER1803-20
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human DDIT3 aa 60-130 |
| Positive control: | Mouse brain tissue lysate, LOVO, mouse testis tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P35638 Human | P35639 Mouse |
| Alternative names: | C/EBP homologous protein C/EBP Homology Protein C/EBP zeta C/EBP-homologous protein 10 C/EBP-homologous protein CCAAT/enhancer binding protein homologous protein CEBPZ CHOP 10 CHOP CHOP-10 CHOP10 DDIT 3 DDIT-3 Ddit3 DDIT3_HUMAN DNA Damage Inducible Transcript 3 DNA damage-inducible transcript 3 protein GADD 153 GADD153 Growth Arrest and DNA Damage Inducible Protein 153 Growth arrest and DNA damage inducible protein GADD153 Growth arrest and DNA damage-inducible protein GADD153 MGC4154 |
Images
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Fig1:
Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ER1803-20) at 1/5,000 dilution. Lane 1: C2C12 (Mouse myoblast) cell lysate Lane 2: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-20, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 19.2 kDa Observed band size: 27 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.