Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 19 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human DDIT3 aa 60-130 |
Positive control: | Mouse brain tissue lysate, LOVO, mouse testis tissue lysate. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
Uniprot #: | SwissProt: P35638 Human | P35639 Mouse |
Alternative names: | C/EBP homologous protein C/EBP Homology Protein C/EBP zeta C/EBP-homologous protein 10 C/EBP-homologous protein CCAAT/enhancer binding protein homologous protein CEBPZ CHOP 10 CHOP CHOP-10 CHOP10 DDIT 3 DDIT-3 Ddit3 DDIT3_HUMAN DNA Damage Inducible Transcript 3 DNA damage-inducible transcript 3 protein GADD 153 GADD153 Growth Arrest and DNA Damage Inducible Protein 153 Growth arrest and DNA damage inducible protein GADD153 Growth arrest and DNA damage-inducible protein GADD153 MGC4154 |
Fig1:
Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: LOVO Lane 3: Mouse brain tissue |
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Fig2: Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |