RYR2 Rabbit Polyclonal Antibody
cat.: ER1803-22
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 565 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RYR2 aa 1,471-1,520 / 4,967. |
| Positive control: | 293T, LOVO, SH-SY-5Y, Siha, human fetal skeletal muscle tissue, mouse brain tissue, mouse cerebellum tissue, rat skeletal muscle tissue. |
| Subcellular location: | Membrane. Sarcoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q92736 Human | E9Q401 Mouse | B0LPN4 Rat |
| Alternative names: | Arrhythmogenic right ventricular dysplasia 2 ARVC2 ARVD2 Brain ryanodine receptor calcium release channel Brain type ryanodine receptor Cardiac muscle ryanodine receptor calcium release channel Cardiac muscle type ryanodine receptor CCO Central core disease of muscle HBRR hRYR 2 hRYR2 MHS MHS1 Ryanodine receptor 1 (skeletal) Ryanodine receptor 1 Ryanodine receptor 2 (cardiac) Ryanodine receptor 2 Ryanodine receptor 3 Ryanodine receptor type1 RYDR RYR 1 RYR 2 RYR 3 RYR RYR-1 RyR1 RYR1_HUMAN RYR2 RYR3 Sarcoplasmic reticulum calcium release channel Skeletal muscle calcium release channel Skeletal muscle ryanodine receptor Skeletal muscle type ryanodine receptor Skeletal muscle-type ryanodine receptor SKRR Type 1 like ryanodine receptor Type 1 ryanodine receptor VTSIP |
Images
|
Fig1: Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500. |
|
Fig2: ICC staining RYR2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining RYR2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4:
Immunocytochemistry analysis of Siha cells labeling RYR2 with Rabbit anti-RYR2 antibody (ER1803-22) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RYR2 antibody (ER1803-22) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig5:
Immunocytochemistry analysis of SH-SY-5Y cells labeling RYR2 with Rabbit anti-RYR2 antibody (ER1803-22) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RYR2 antibody (ER1803-22) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR2 antibody. Counter stained with hematoxylin. |
|
Fig9: Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.