KCNA5 Rabbit Polyclonal Antibody
cat.: ER1803-23
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat KCNA5 aa 249-298 / 602. |
| Positive control: | PANC-1 cell lysate, human brain tissue lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, rat skeletal muscle tissue, rat heart tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:800 |
| Uniprot #: | SwissProt: P22460 Human | Q61762 Mouse | P19024 Rat |
| Alternative names: | ATFB7 cardiac potassium channel HCK1 HK2 HPCN1 insulinoma and islet potassium channel KCNA5 KCNA5_HUMAN KV1.5 PCN1 potassium channel 1 Potassium channel, insulinoma and islet cell Potassium channel, voltage-gated, shaker-related subfamily, member 5 Potassium voltage gated channel subfamily A member 5 Potassium voltage-gated channel subfamily A member 5 Voltage-gated potassium channel HK2 voltage-gated potassium channel protein Kv1.5 Voltage-gated potassium channel subunit Kv1.5 |
Images
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Fig1:
Western blot analysis of KCNA5 on different lysates with Rabbit anti-KCNA5 antibody (ER1803-23) at 1/2,000 dilution. Lane 1: PANC-1 cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: Lane 1-3: 14 seconds; Lane 4: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-KCNA5 antibody (ER1803-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-23) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-KCNA5 antibody (ER1803-23) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-23) at 1/800dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.