Anti-KCNA5 antibody
cat.: ER1803-23
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 67 kDa
Isotype: IgG
Immunogen: Synthetic peptide within rat KCNA5 aa 250-300.
Positive control: PANC-1, LOVO, SKOV-3, rat skeletal muscle tissue, rat heart tissue.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P22460 Human | Q61762 Mouse | P19024 Rat
Alternative names: ATFB7 cardiac potassium channel HCK1 HK2 HPCN1 insulinoma and islet potassium channel KCNA5 KCNA5_HUMAN KV1.5 PCN1 potassium channel 1 Potassium channel, insulinoma and islet cell Potassium channel, voltage-gated, shaker-related subfamily, member 5 Potassium voltage gated channel subfamily A member 5 Potassium voltage-gated channel subfamily A member 5 Voltage-gated potassium channel HK2 voltage-gated potassium channel protein Kv1.5 Voltage-gated potassium channel subunit Kv1.5
Images
ER1803-23_1.jpg Fig1: Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1/5,000 dilution.
ER1803-23_2.jpg Fig2: ICC staining KCNA5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-23_3.jpg Fig3: ICC staining KCNA5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-23_4.jpg Fig4: ICC staining KCNA5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-23_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.
ER1803-23_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.
ER1803-23_7.jpg Fig7: Flow cytometric analysis of PANC-1 cells with KCNA5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.