CD42b Rabbit Polyclonal Antibody
cat.: ER1803-26
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD42b aa 117-328 / 652. |
| Positive control: | Jurkat, human spleen tissue. |
| Subcellular location: | Membrane. Secreted. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: P07359 Human |
| Alternative names: | Antigen CD42b alpha BDPLT1 BDPLT3 BSS CD 42b CD42b alpha CD42B CD42b antigen DBPLT3 GLYCOCALICIN Glycoprotein Ib (platelet) alpha polypeptide Glycoprotein Ibalpha GP Ib alpha GP1B GP1BA GPIb alpha GPIbA MGC34595 Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib alpha polypeptide Platelet membrane glycoprotein 1b alpha subunit VWDP |
Images
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Fig1: ICC staining CD42b in Jurkat cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD42b antibody (ER1803-26) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-26) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Jurkat cells with CD42b antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.