| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CACNG5 aa 68-117 / 275. |
| Positive control: | HepG2, LOVO, PANC-1. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:100 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UF02 Human | Q8VHW8 Rat |
| Alternative names: | CACNG 5 Cacng5 Calcium channel voltage dependent gamma subunit 5 CCG5_HUMAN MGC126656 MGC126682 Neuronal voltage gated calcium channel gamma 5 subunit Neuronal voltage-gated calcium channel gamma-5 subunit Voltage dependent calcium channel gamma 5 subunit Voltage-dependent calcium channel gamma-5 subunit |
|
Fig1: Western blot analysis of CACNG5 on LOVO cell lysate using anti-CACNG5 antibody at 1/200 dilution. |
|
Fig2: ICC staining CACNG5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CACNG5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CACNG5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of PANC-1 cells with CACNG5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |