CACNB1 Rabbit Polyclonal Antibody
cat.: ER1803-29
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 66/58/53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse CACNB1 aa 439-488 / 597.
Positive control: SH-SY-5Y, rat heart tissue, human tonsil tissue, human kidney tissue, human fetal skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell

1:500-1:1,000
1:50-1:200
1:50
Uniprot #: SwissProt: Q02641 Human | Q8R3Z5 Mouse | P54283 Rat
Alternative names: CAB1 CACB1_HUMAN CACNB1 CACNLB1 Calcium channel L type beta 1 polypeptide Calcium channel voltage dependent beta 1 subunit Calcium channel voltage-dependent subunit beta 1 CCHLB1 Dihydropyridine sensitive L type calcium channel beta 1 subunit MGC41896 Voltage dependent L type calcium channel beta 1 subunit Voltage-dependent L-type calcium channel subunit beta-1
Images
ER1803-29_1.jpg Fig1: Western blot analysis of CACNB1 on SH-SY-5Y cell lysate using anti-CACNB1 antibody at 1/1,000 dilution.
Lane 1: SH-SY-5Y cell lysate
Lane 2: SH-SY-5Y cell lysate with immunizaiton peptide
ER1803-29_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
ER1803-29_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
ER1803-29_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
ER1803-29_5.png Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
ER1803-29_6.png Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-CACNB1 antibody (ER1803-29) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.