Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 66/58/53 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse CACNB1 aa 439-488 / 597. |
Positive control: | SH-SY-5Y, rat heart tissue, human tonsil tissue, human kidney tissue, human fetal skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:50-1:200 1:50 |
Uniprot #: | SwissProt: Q02641 Human | Q8R3Z5 Mouse | P54283 Rat |
Alternative names: | CAB1 CACB1_HUMAN CACNB1 CACNLB1 Calcium channel L type beta 1 polypeptide Calcium channel voltage dependent beta 1 subunit Calcium channel voltage-dependent subunit beta 1 CCHLB1 Dihydropyridine sensitive L type calcium channel beta 1 subunit MGC41896 Voltage dependent L type calcium channel beta 1 subunit Voltage-dependent L-type calcium channel subunit beta-1 |
Fig1:
Western blot analysis of CACNB1 on SH-SY-5Y cell lysate using anti-CACNB1 antibody at 1/1,000 dilution. Lane 1: SH-SY-5Y cell lysate Lane 2: SH-SY-5Y cell lysate with immunizaiton peptide |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNB1 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin. | |
Fig6:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-CACNB1 antibody (ER1803-29) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |