|Product Type:||Rabbit polyclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse|
Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysates
Lane 2: Mouse placenta tissue lysates