Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 37 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse IL12B aa 220-300. |
Positive control: | Hela, Mouse placenta tissue lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: P29460 Human | P43432 Mouse |
Alternative names: | CLMF CLMF p40 CLMF2 Cytotoxic lymphocyte maturation factor 40 kDa subunit IL 12B IL-12 subunit p40 IL-12B IL12 p40 IL12B IL12B_HUMAN IMD28 IMD29 Interleukin 12 beta chain Interleukin 12 p40 Interleukin-12 subunit beta NK cell stimulatory factor chain 2 NKSF NKSF2 |
Fig1:
Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysates Lane 2: Mouse placenta tissue lysates |