HKDC1 Rabbit Polyclonal Antibody
cat.: ER1803-32
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 82/90/102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HKDC1 aa 266-462 / 917. |
| Positive control: | LOVO, MCF-7, SiHa, rat kidney tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Mitochondrion membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q2TB90 Human | Q91W97 Mouse | M0R3X7 Rat |
| Alternative names: | BC016235 Hexokinase domain containing 1 Hexokinase domain-containing protein 1 Hkdc1 HKDC1_HUMAN Putative hexokinase HKDC1 |
Images
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Fig1: ICC staining HKDC1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining HKDC1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining HKDC1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HKDC1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HKDC1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.