ATP2A1 Rabbit Polyclonal Antibody
cat.: ER1803-34
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 110 kDa
Isotype: IgG
Immunogen: Synthetis peptide within mouse ATP2A1 aa 1-50 / 994.
Positive control: Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, 293T.
Subcellular location: Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O14983 Human | Q8R429 Mouse | Q64578 Rat
Alternative names: fast twitch skeletal muscle isoform AT2A1_HUMAN ATP2A ATP2A1 ATPase Ca++ transporting cardiac muscle fast twitch 1 ATPase Ca++ transporting fast twitch 1 ATPase, Ca(2+)-transporting fast twitch 1 Calcium pump 1 Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform Calcium-transporting ATPase sarcoplasmic reticulum type EC 3.6.3.8 Endoplasmic reticulum class 1/2 Ca(2+) ATPase Fast skeletal muscle SR calcium ATPase OTTHUMP00000162561 OTTHUMP00000162562 Sarcoendoplasmic reticulum calcium ATPase Sarcoplasmic reticulum Ca(2+)-ATPase 1 Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 SERCA 1 SERCA1 SERCA1 truncated isoform, included SR Ca(2+) ATPase 1 SR Ca(2+)-ATPase 1
Images
ER1803-34_1.jpg Fig1: Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse skeletal muscle tissue
Lane 2: Human skeletal muscle tissue
ER1803-34_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
ER1803-34_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
ER1803-34_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.
ER1803-34_5.jpg Fig5: Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.