Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 110 kDa |
Isotype: | IgG |
Immunogen: | Synthetis peptide within mouse ATP2A1 aa 1-50 / 994. |
Positive control: | Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, 293T. |
Subcellular location: | Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: O14983 Human | Q8R429 Mouse | Q64578 Rat |
Alternative names: | fast twitch skeletal muscle isoform AT2A1_HUMAN ATP2A ATP2A1 ATPase Ca++ transporting cardiac muscle fast twitch 1 ATPase Ca++ transporting fast twitch 1 ATPase, Ca(2+)-transporting fast twitch 1 Calcium pump 1 Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform Calcium-transporting ATPase sarcoplasmic reticulum type EC 3.6.3.8 Endoplasmic reticulum class 1/2 Ca(2+) ATPase Fast skeletal muscle SR calcium ATPase OTTHUMP00000162561 OTTHUMP00000162562 Sarcoendoplasmic reticulum calcium ATPase Sarcoplasmic reticulum Ca(2+)-ATPase 1 Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 SERCA 1 SERCA1 SERCA1 truncated isoform, included SR Ca(2+) ATPase 1 SR Ca(2+)-ATPase 1 |
Fig1:
Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse skeletal muscle tissue Lane 2: Human skeletal muscle tissue |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |