AMBP Rabbit Polyclonal Antibody
cat.: ER1803-35
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AMBP aa 120-320. |
| Positive control: | HepG2 cell lysate, A549 cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, HepG2, human liver tissue. |
| Subcellular location: | Secreted, Cytoplasm, cytosol, Cell membrane, Nucleus membrane, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:500 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P02760 Human | Q07456 Mouse | Q64240 Rat |
| Alternative names: | Alpha 1 microglobulin/bikunin precursor Alpha-1 microglycoprotein AMBP AMBP_HUMAN Bikunin Complex-forming glycoprotein heterogeneous in charge EDC1 Growth inhibiting protein 19 HI 30 HI-30 HI30 IATIL Inter alpha trypsin inhibitor light chain ITI ITI LC ITI-LC ITILC Protein HC Trypstatin Uronic-acid-rich protein UTI |
Images
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Fig1:
Western blot analysis of AMBP on different lysates with Rabbit anti-AMBP antibody (ER1803-35) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat liver tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 39 kDa Observed band size: 55 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling AMBP with Rabbit anti-AMBP antibody (ER1803-35) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMBP antibody (ER1803-35) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AMBP antibody (ER1803-35) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling AMBP. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-35, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.