AMBP Rabbit Polyclonal Antibody
cat.: ER1803-35
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AMBP aa 120-320. |
| Positive control: | Human liver, Mouse liver, Mouse brain, Hela, HepG2, MCF-7, human liver tissue, human liver cancer tissue, human kidney tissue. |
| Subcellular location: | Secreted, Cytoplasm, cytosol, Cell membrane, Nucleus membrane, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P02760 Human | Q07456 Mouse |
| Alternative names: | Alpha 1 microglobulin/bikunin precursor Alpha-1 microglycoprotein AMBP AMBP_HUMAN Bikunin Complex-forming glycoprotein heterogeneous in charge EDC1 Growth inhibiting protein 19 HI 30 HI-30 HI30 IATIL Inter alpha trypsin inhibitor light chain ITI ITI LC ITI-LC ITILC Protein HC Trypstatin Uronic-acid-rich protein UTI |
Images
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Fig1:
Western blot analysis of AMBP on different cell lysates using anti-AMBP antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Human liver Lane 3: Mouse liver Lane 4: Mouse brain |
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Fig2: ICC staining AMBP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining AMBP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining AMBP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AMBP antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AMBP antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMBP antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of HepG2 cells with AMBP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.