Anti-PHF8 antibody
cat.: ER1803-37
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 118 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PHF8 aa 150-350.
Positive control: PC-3M, A431, Hela, JAR, rat brain tissue , human lung cancer tissue, human colon tissue, mouse brain tissue, human kidney tissue, Siha.
Subcellular location: Nuclear membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UPP1 Human
Alternative names: Histone lysine demethylase PHF8 JHDM1F Jumonji C domain containing histone demethylase 1F MRXSSD PHD finger protein 8 PHF8 PHF8_HUMAN ZNF422
Images
ER1803-37_1.jpg Fig1: Western blot analysis of PHF8 on different cell lysate using anti-PHF8 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: A431
Lane 3: Human kidney tissue
ER1803-37_2.jpg Fig2: ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-37_3.jpg Fig3: ICC staining PHF8 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-37_4.jpg Fig4: ICC staining PHF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-37_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.
ER1803-37_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHF8 antibody. Counter stained with hematoxylin.
ER1803-37_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PHF8 antibody. Counter stained with hematoxylin.
ER1803-37_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.
ER1803-37_9.jpg Fig9: Flow cytometric analysis of SiHa cells with PHF8 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.