PHF8 Rabbit Polyclonal Antibody
cat.: ER1803-37
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 118 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PHF8 aa 153-362 / 1,060. |
| Positive control: | A431 cell lysates, Hela, JAR, rat brain tissue , human lung cancer tissue, human colon tissue, mouse brain tissue, human kidney tissue, Siha. |
| Subcellular location: | Nuclear membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UPP1 Human |
| Alternative names: | Histone lysine demethylase PHF8 JHDM1F Jumonji C domain containing histone demethylase 1F MRXSSD PHD finger protein 8 PHF8 PHF8_HUMAN ZNF422 |
Images
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Fig1:
Western blot analysis of PHF8 on A431 cell lysates with Rabbit anti-PHF8 antibody (ER1803-37) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 94 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-37, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 118 kDa Observed band size: 140 kDa |
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Fig2: ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PHF8 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PHF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHF8 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PHF8 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.