CACNG3 Rabbit Polyclonal Antibody
cat.: ER1803-38
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat CACNG3 aa 192-241 / 315. |
| Positive control: | SH-SY-5Y, rat brain tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O60359 Human | Q8VHX0 Rat |
| Alternative names: | CACNG 3 Cacng2 CACNG3 Calcium channel voltage dependent gamma subunit 3 CCG3_HUMAN Neuronal voltage gated calcium channel gamma 3 subunit Neuronal voltage-gated calcium channel gamma-3 subunit TARP gamma-3 Transmembrane AMPAR regulatory protein gamma-3 Voltage dependent calcium channel gamma 3 subunit Voltage gated calcium channel gamma subunit Voltage-dependent calcium channel gamma-3 subunit |
Images
|
Fig1:
Western blot analysis of CACNG3 on different lysates with Rabbit anti-CACNG3 antibody (ER1803-38) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-38, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 50 kDa |
|
Fig2: ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.