| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat CACNG3 aa 192-241 / 315. |
| Positive control: | SH-SY-5Y, rat brain tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O60359 Human | Q8VHX0 Rat |
| Alternative names: | CACNG 3 Cacng2 CACNG3 Calcium channel voltage dependent gamma subunit 3 CCG3_HUMAN Neuronal voltage gated calcium channel gamma 3 subunit Neuronal voltage-gated calcium channel gamma-3 subunit TARP gamma-3 Transmembrane AMPAR regulatory protein gamma-3 Voltage dependent calcium channel gamma 3 subunit Voltage gated calcium channel gamma subunit Voltage-dependent calcium channel gamma-3 subunit |
|
Fig1:
Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody. Lane 1: Anti-CACNG3 Antibody (1:500). Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide. |
|
Fig2: ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin. |
|
Fig4: Flow cytometric analysis of SH-SY-5Y cells with CACNG3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |