KCNK1 Rabbit Polyclonal Antibody
cat.: ER1803-39
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human KCNK1 aa 40-100. |
| Positive control: | Human placenta tissue, human liver tissue, rat cerebellum tissue, human kidney tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Recycling endosome, Synaptic cell membrane, Cytoplasmic vesicle, Perikaryon, Cell projection, dendrite, Apical cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1ug/mL |
| Uniprot #: | SwissProt: O00180 Human | O08581 Mouse | Q9Z2T2 Rat |
| Alternative names: | DPK HOHO HOHO1 inward rectifying potassium channel protein TWIK 1 Inward rectifying potassium channel protein TWIK-1 Inward rectifying potassium channel protein TWIK1 K2P1 K2p1.1 KCNK1 KCNK1_HUMAN KCNO1 OTTHUMP00000036029 Potassium channel KCNO1 Potassium channel subfamily K member 1 Potassium inwardly rectifying channel subfamily K member 1 TWIK 1 TWIK1 |
Images
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Fig1:
Western blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution. Positive control: Lane 1: Human placenta Lane 2: Human liver |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KCNK1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KCNK1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNK1 antibody. Counter stained with hematoxylin. |
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Fig7:
Flow cytometric analysis of SH-SY5Y cells labeling KCNK1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-39, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.