KCNK1 Rabbit Polyclonal Antibody
cat.: ER1803-39
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human KCNK1 aa 40-100.
Positive control: Human placenta tissue, human liver tissue, rat cerebellum tissue, human kidney tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Cell membrane, Recycling endosome, Synaptic cell membrane, Cytoplasmic vesicle, Perikaryon, Cell projection, dendrite, Apical cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:50-1:200
1ug/mL
Uniprot #: SwissProt: O00180 Human | O08581 Mouse | Q9Z2T2 Rat
Alternative names: DPK HOHO HOHO1 inward rectifying potassium channel protein TWIK 1 Inward rectifying potassium channel protein TWIK-1 Inward rectifying potassium channel protein TWIK1 K2P1 K2p1.1 KCNK1 KCNK1_HUMAN KCNO1 OTTHUMP00000036029 Potassium channel KCNO1 Potassium channel subfamily K member 1 Potassium inwardly rectifying channel subfamily K member 1 TWIK 1 TWIK1
Images
ER1803-39_1.jpg Fig1: Western blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Human placenta
Lane 2: Human liver
ER1803-39_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.
ER1803-39_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.
ER1803-39_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.
ER1803-39_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.
ER1803-39_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.
ER1803-39_7.jpg Fig7: Flow cytometric analysis of SH-SY5Y cells labeling KCNK1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-39, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.