LOXL2 Rabbit Polyclonal Antibody
cat.: ER1803-41
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LOXL2 aa 300-480. |
| Positive control: | U-87 MG (Human glioblastoma cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, A549, SiHa, rat testis tissue, human placenta tissue, human uterus tissue, mouse testis tissue. |
| Subcellular location: | Endoplasmic reticulum. Nucleus. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000-1:10,000 1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y4K0 Human | P58022 Mouse | B5DF27 Rat |
| Alternative names: | LOR 2 LOR2 LOX L2 LOXL 2 LOXL2 LOXL2_HUMAN Lysyl oxidase homolog 2 Lysyl oxidase like 2 Lysyl oxidase like protein 2 Lysyl oxidase related 2 Lysyl oxidase related protein 2 Lysyl oxidase related protein WS9 14 Lysyl oxidase-like protein 2 Lysyl oxidase-related protein 2 Lysyl oxidase-related protein WS9-14 WS9 14 |
Images
|
Fig1:
Western blot analysis of LOXL2 on different lysates with Rabbit anti-LOXL2 antibody (ER1803-41) at 1/10,000 dilution. Lane 1: U-87 MG (Human glioblastoma cell) cell lysate (10 µg/Lane) Lane 2: HUVEC (Human umbilical vein endothelial cell) cell lysate (10 µg/Lane) Lane 3: MCF7 (Human breast cancer cell) cell lysate (30 µg/Lane) Exposure time: 2 minutes 7 seconds; ECL: K1801 Low expression of LOXL2 protein in MCF7 is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-41, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 87 kDa Observed band size: 87 kDa |
|
Fig2: ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.