LOXL2 Rabbit Polyclonal Antibody
cat.: ER1803-41
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LOXL2 aa 300-480. |
| Positive control: | A431, A549, SiHa, rat testis tissue, human placenta tissue, human uterus tissue, mouse testis tissue. |
| Subcellular location: | Endoplasmic reticulum. Nucleus. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y4K0 Human | P58022 Mouse | B5DF27 Rat |
| Alternative names: | LOR 2 LOR2 LOX L2 LOXL 2 LOXL2 LOXL2_HUMAN Lysyl oxidase homolog 2 Lysyl oxidase like 2 Lysyl oxidase like protein 2 Lysyl oxidase related 2 Lysyl oxidase related protein 2 Lysyl oxidase related protein WS9 14 Lysyl oxidase-like protein 2 Lysyl oxidase-related protein 2 Lysyl oxidase-related protein WS9-14 WS9 14 |
Images
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Fig1: Western blot analysis of LOXL2 on A549 and A431 cell lysate using anti-LOXL2 antibody at 1/500 dilution. |
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Fig2: ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
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Fig8: Flow cytometric analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.