Calnexin Rabbit Polyclonal Antibody
cat.: ER1803-42
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Calnexin aa 555-592/592. |
| Positive control: | Daudi cell lysate, mouse lung tissue lysate, human kidney tissue, SKOV-3, Hela cell lysate, rat lung tissue lysate, rat brain tissue lysate, rat brain tissue, human brain tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:2,000- 1:10,000 1:200-1:500 1:50-1:100 1-2μg/sample |
| Uniprot #: | SwissProt: P27824 Human | P35564 Mouse |
| Alternative names: | Calnexin CALX_HUMAN CANX CNX FLJ26570 Histocompatibility complex class I antigen binding protein p88 IP90 Major histocompatibility complex class I antigen-binding protein p88 p90 |
Images
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Fig1:
Western blot analysis of Calnexin on different lysates with Rabbit anti-Calnexin antibody (ER1803-42) at 1/5,000 dilution. Lane 1: Daudi cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-42) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Calnexin on different lysates with Rabbit anti-Calnexin antibody (ER1803-42) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Calnexin KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 90 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-42) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of Calnexin on different lysates with Rabbit anti-Calnexin antibody (ER1803-42) at 1/2,000 dilution. Lane 1: Hela cell lysate（10 µg/Lane） Lane 2: Rat lung tissue lysate（20 µg/Lane） Lane 3: Rat brain tissue lysate（20 µg/Lane） Predicted band size: 68 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-42) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calnexin antibody (ER1803-42) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-42) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Calnexin antibody (ER1803-42) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Calnexin antibody (ER1803-42) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Calnexin antibody (ER1803-42) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Flow cytometric analysis of SKOV-3 cells labeling Calnexin. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-42, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig9:
Calnexin was immunoprecipitated from 0.2 mg HeLa cell lysate with ER1803-42 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ER1803-42 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ER1803-42 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ER1803-42 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 23 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.