APR3 Rabbit Polyclonal Antibody
cat.: ER1803-44
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse APR3 aa 174-223 / 223. |
| Positive control: | Human skin tissue lysates, A549, SH-SY-5Y, mouse kidney tissue, MCF-7. |
| Subcellular location: | Nucleus envelope, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-2,000 1:50-1:200 1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q6UW56 Human | Q6PGD0 Mouse | B2RYU3 Rat |
| Alternative names: | APR-3 ATRAID APR3 C2orf28 HSPC013 UNQ214/PRO240 |
Images
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Fig1:
Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody. Lane 1: Anti-APR3 antibody (1/500). Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide. |
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Fig2: ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-APR3 antibody (ER1803-44) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-44) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.