Methyltransferase-like 26 Rabbit Polyclonal Antibody
cat.: ER1803-45
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Methyltransferase-like 26 aa 33-82 / 204. |
| Positive control: | Mouse kidney tissue lysate, Mouse pancreas tissue lysate, SH-SY-5Y, MCF-7, human lung tissue, mouse kidney tissue, mouse heart tissue, human thyroid cancer tissue, mouse small intestine tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:20,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96S19 Human | Q9DCS2 Mouse | Q497C3 Rat |
| Alternative names: | 2610100D03Rik 2810013M15Rik A830095F14Rik AI846133 Methyltransferase 10 domain containing Methyltransferase like 16 METT10D MGC3329 OTTMUSP00000006473 Putative methyltransferase METT10D RP23-174M12.2 |
Images
|
Fig1:
Western blot analysis of Methyltransferase-like 26 on different lysates with Rabbit anti-Methyltransferase-like 26 antibody (ER1803-45) at 1/20,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse pancreas tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-45, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22.5 kDa Observed band size: 22.5 kDa |
|
Fig2: ICC staining Methyltransferase-like 26 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Methyltransferase-like 26 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Methyltransferase-like 26 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Methyltransferase-like 26 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Methyltransferase-like 26 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using anti-Methyltransferase-like 26 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Methyltransferase-like 26 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.