Nfic Rabbit Polyclonal Antibody
cat.: ER1803-46
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Nfic aa 390-439 / 439.
Positive control: A549 cell lysate, SiHa cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P08651 Human
Alternative names: 1110019L22Rik 1500041O16Rik AA589446 AI746521 CAAT box transcription factor CCAAT binding transcription factor CCAAT box binding transcription factor CCAAT-box-binding transcription factor CNFI C CTF CTF5 MGC137374 MGC20153 NF I NF I/C NF-I/C NF1 C NF1-C NF1C NFI NFI-C NFI/C NFIC NFIC_HUMAN Nuclear factor 1 Nuclear factor 1 C type Nuclear factor 1 C-type Nuclear factor 1/C Nuclear factor I/C (CCAAT binding transcription factor) Nuclear factor I/C TGGCA binding protein TGGCA-binding protein Transcription factor NFIC
Images
ER1803-46_1.jpg Fig1: Western blot analysis of Nfic on different lysates with Rabbit anti-Nfic antibody (ER1803-46) at 1/500 dilution.

Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-46) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.