| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Nfic aa 390-439 / 439. |
| Positive control: | A549 cell lysate, SiHa cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P08651 Human |
| Alternative names: | 1110019L22Rik 1500041O16Rik AA589446 AI746521 CAAT box transcription factor CCAAT binding transcription factor CCAAT box binding transcription factor CCAAT-box-binding transcription factor CNFI C CTF CTF5 MGC137374 MGC20153 NF I NF I/C NF-I/C NF1 C NF1-C NF1C NFI NFI-C NFI/C NFIC NFIC_HUMAN Nuclear factor 1 Nuclear factor 1 C type Nuclear factor 1 C-type Nuclear factor 1/C Nuclear factor I/C (CCAAT binding transcription factor) Nuclear factor I/C TGGCA binding protein TGGCA-binding protein Transcription factor NFIC |
|
Fig1:
Western blot analysis of Nfic on different lysates with Rabbit anti-Nfic antibody (ER1803-46) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Exposure time: 25 seconds ; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-46, 1/5,000 in primary antibody dilution buffer (K1803), 2 hour at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 56 kDa Observed band size: 56 kDa |