CaV2.3 Rabbit Polyclonal Antibody
cat.: ER1803-47
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 262 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat CaV23 aa 875-924 / 2,222. |
| Positive control: | Rat brain tissue lysate, LOVO, rat brain tissue, human placenta tissue, mouse testis tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q15878 Human | Q61290 Mouse | Q07652 Rat |
| Alternative names: | alpha-1 polypeptide BII Brain calcium channel II CAC 1E CAC1E CAC1E_HUMAN CACH 6 CACH6 CACNA1E Cacnl1 a6 CACNL1A6 Calcium channel Calcium channel, L type, alpha 1 polypeptide, isoform 6 calcium channel, R type Cav 2.3 Cchra 1 Cchra1 isoform 6 L type Voltage dependent R type calcium channel subunit alpha 1E Voltage gated calcium channel subunit alpha Cav2.3 Voltage-dependent R-type calcium channel subunit alpha-1E Voltage-gated calcium channel subunit alpha Cav2.3 |
Images
|
Fig1:
Western blot analysis of CaV2.3 on different lysates with Rabbit anti-CaV2.3 antibody (ER1803-47) at 1/2,000 dilution. Lane 1: Rat brain tissue lysate (unboiled) Lane 2: Rat kidney tissue lysate (unboiled) Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative expression of CaV2.3 protein in kidney is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-47, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 252 kDa Observed band size: 270 kDa |
|
Fig2: ICC staining CaV2.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.