CACNA1C Rabbit Polyclonal Antibody
cat.: ER1803-49
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: Dot Blot, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 249 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse CACNA1C aa 802-851 / 2,139.
Positive control: SiHa, SKOV-3, rat brain tissue, human kidney tissue, human uterus tissue, mouse heart tissue.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  Dot Blot
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q13936 Human | Q01815 Mouse | P22002 Rat
Alternative names: alpha-1 polypeptide cardiac muscle isoform 1 L type CAC1C_HUMAN CACH 2 CACH2 CACN 2 CACN2 CACNA1C CACNL1A1 Calcium channel Calcium channel cardic dihydropyridine sensitive alpha 1 subunit Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle Calcium channel voltage dependent L type alpha 1C subunit CaV1.2 CCHL1A1 DHPR alpha 1 DHPR alpha 1 subunit LQT8 TS Voltage dependent L type calcium channel alpha 1C subunit Voltage dependent L type calcium channel subunit alpha 1C Voltage gated calcium channel alpha subunit Cav1.2 Voltage gated calcium channel subunit alpha Cav1.2 Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* Voltage-dependent L-type calcium channel subunit alpha-1C Voltage-gated calcium channel subunit alpha Cav1.2
Images
ER1803-49_1.jpg Fig1: Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.
ER1803-49_2.jpg Fig2: ICC staining of CACNA1C in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1803-49, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1803-49_3.jpg Fig3: ICC staining of CACNA1C in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1803-49, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1803-49_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNA1C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-49_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CACNA1C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-49_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-CACNA1C antibody (ER1803-49) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-49) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-49_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CACNA1C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-49_8.jpg Fig8: Flow cytometric analysis of CACNA1C was done on SKOV-3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1803-49, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
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