| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Avian avulavirus 1 |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within newcastle disease virus Hemagglutinin-neuraminidase aa 206-255 / 586. |
| Positive control: | Hemagglutinin-neuraminidase transfected HEK293 cell. |
| Subcellular location: | Virion membrane. |
| Recommended Dilutions:
WB ELISA |
1:500-1,000 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: B5UA17 Avian avulavirus 1 |
|
Fig1:
Western blot analysis of Hemagglutinin-neuraminidase on HEK293 cell lysates with Rabbit anti-Hemagglutinin-neuraminidase antibody (ER1803-50) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-50, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 64 kDa Observed band size: 64 kDa |